Sordaria Lab Report

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Introduction: Sordaria fimicola is an ascomycete fungus which is a haploid organism for most of its life cycle (Alexopoulos et al. 1996). This fungus produces black perithecia containing asci with eight dark ascospores in a linear arrangement. Within an ascus each zygote undergoes meiosis and gives rise to four haploid cells. After meiosis the cells then undergo one round of mitotic division which gives rise to a total of eight haploid ascospores. Although S. fimicola is self fertile they can be crossed. It is the sexual phase where two haploid strains can fuse to form diploid zygotes (Mertens, 2001). These diploid zygotes are encased in the ascus. If two mycelia meet the two haploid nuclei can fuse to form a heterozygous diploid. A marker color mutant can be employed to detect these…show more content…
The long, thin ascus causes both meiotic spindles and the mitotic spindles to be aligned during spore formation. As a result, the positioning of the ascospores directly reflects the arrangement of homologous chromosome pairs during metaphase I of meiosis (Glase, 1995). This allows us to detect which alleles were exchanged creating crossovers. Sexual reproduction in the S. fimicola is important because an it allows for a significant source of genetic variability, and allows the fungus to adapt to new environments (Fincham, 1971). This genetic variability is obtained when crossovers occur between

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