Microbiology Lab Lag Phase

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Introduction The term “growth” has many definitions to microbiologists. It often means an increase in cellular constituents and thus the concept of a cell growing longer and larger, while undergoing different forms of cell division mainly binary fission. Therefore growth can also refer to the growth in size of a population. Microorganisms follow a trend throughout their life cycle. One beneficial method of showing this trend is by using a microbial growth curve. There are four phases of this curve; the lag phase, the exponential phase, the stationary phase and the death phase. The Lag phase is the first phase of the curve and is often called an adjustment period. This is because there is no immediate increase in cell number, but cells are synthesizing new components. This phase is important for cells to repair any damaged components and produce any new necessary enzymes needed to thrive in the given medium. The Exponential Phase is where maximum growth occurs given their genetic potential, nature of the given medium and the environmental conditions. The Stationary Phase that is reached by most bacteria at a population of 109 cells per millimeter. This is the phase in which the cell balances between the growth rate and the death rate. And finally The Death Phase which is where microbial death occurs which is the reverse of microbial growth. The importance of evaluating the growth rate of microbes is to discover bactericidal or antibiotic concentrations, temperature and pH at which they stop growing. It gives an idea of generation time and the mean growth rate constant that allows for the minimum, maximum and optimum temperature of a microbe to be calculated. This lab aims to obtain a microbial growth curve of a batch of Saccharomyces cervisiae through the use of optical density measurements and cell densities obtained from plate counts and direct

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